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The contents of this book are as follows: Chapter1:Pre-processing steps for mouse experiments 1.1 Experimental mouse species 1.2 Gene knockout mouse 1.3 mouse holder 1.4 Mouse feed and raise 1.5 Sacrifice Chapter2:Preparation of mice before surgery 2.1 Anesthetics for mice 2.2 Mouse Surgical Instrument Selection Table 2.3 Blood Collection Techniques in Mice Chapter3 Applied Molecular biology 3.1 Western Blot 3.2 Mouse Tissue Cell Culture 3.3 Immunohistochemistry(IHC) 3.4 RNA extraction 3.5 cryosectioning